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The objective of the current study was to investigate the antioxidant, anti-inflammatory, and analgesic activities of the ethanolic extract from the fruits of Ficus heteorphylla var. assamica (FHVAEE). Several in-vitro tests were performed on the plant's fruit ethanol extract. The antioxidant capability of diverse compounds was assessed using tests for total phenol content, total flavonoid content, DPPH free radical scavenging activity, and total antioxidant capacity. Protein denaturation was tested against anti-inflammatory properties. On a test for total phenol content, the fruit extract had a modest antioxidant capability; TPC as GAE was 120 ±3.14 mg/gm. Total flavonoid content assay results indicated exceptional antioxidant activity; TFC as QE was 428.33 ±7.07 mg/gm. The IC50 value for the DPPH free radical scavenging experiment was 61.17 μg/ml, while standard ascorbic acid was found to have an IC50 value of 16.97 μg/ml, indicating modest antioxidant activity. According to the anti-inflammatory assay, the plant fruit extract has excellent anti-inflammatory potential. The presence of several phytoconstituents in the extract could be the cause of these effects. To assess these effects and the results, which call for further advanced research, more studies must be done.

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